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2025 01 v.31 135-141
烟草棒孢霉叶斑病拮抗菌TKY-11的生防潜力评价
基金项目(Foundation): 重点研发项目“基于微生态调控烟草棒孢霉叶斑病绿色防控技术研究与应用”(No.2022XM09)
邮箱(Email): 420991338@qq.com;
DOI: 10.16472/j.chinatobacco.2023.T0143
中文作者单位:

贵州省烟草公司正安县公司;安徽农业大学生命科学学院;贵州省烟草公司遵义市公司;

摘要(Abstract):

【目的】获得对烟草棒孢霉叶斑病有防控效果的微生物菌种。【方法】利用稀释涂布平板法分离纯化烟株内生细菌,筛选出对多主棒孢霉具有较好拮抗活性的内生细菌,结合形态学和分子生物学方法开展拮抗菌株鉴定;采用浸渍法测定拮抗菌对多主棒孢霉菌丝形态、分生孢子萌发和抗氧化酶活性的影响;通过盆栽法评价拮抗菌对烟草棒孢霉叶斑病的防控效果。【结果】(1)从健康烟株根、茎及叶分离获得55株内生细菌,其中TKY-11对多主棒孢霉拮抗效果较好,抑菌率65.00%,鉴定为贝莱斯芽孢杆菌属Bacillus velezensis。(2)TKY-11菌株发酵上清液显著抑制多主棒孢霉分生孢子萌发,处理18 h时孢子萌发率仅50.67%;发酵上清液能降低多主棒孢霉菌丝的抗氧化酶活性,并破坏菌丝细胞结构。(3)接种TKY-11可有效降低烟草棒孢霉叶斑病的发病程度。【结论】本研究分离的贝莱斯芽孢杆菌TKY-11对多主棒孢霉有拮抗作用,具有一定的开发潜力。

关键词(KeyWords): 烟草棒孢霉叶斑病;多主棒孢霉;贝莱斯芽孢杆菌;拮抗作用;防效
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基本信息:

DOI:10.16472/j.chinatobacco.2023.T0143

中图分类号:S435.72;S476

引用信息:

[1]杨相,杨倩,龙星黔等.烟草棒孢霉叶斑病拮抗菌TKY-11的生防潜力评价[J].中国烟草学报,2025,31(01):135-141.DOI:10.16472/j.chinatobacco.2023.T0143.

基金信息:

重点研发项目“基于微生态调控烟草棒孢霉叶斑病绿色防控技术研究与应用”(No.2022XM09)

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